NSI-189 Free Base – Solution

PRODUCT DESCRIPTION

Introduction

NSI-189 Free Base Solution is a liquid formulation of the neurogenic small molecule NSI-189, designed for research purposes. This solution typically contains NSI-189 dissolved at a concentration suitable for experimental use, often around 40 mg/mL. The solution offers enhanced convenience for dosing and handling compared to powder forms. NSI-189 itself is a benzylpiperazine-aminopyridine derivative that stimulates the growth of new neurons (neurogenesis), mainly in the hippocampus region of the brain. It is widely researched for its potential in cognitive enhancement, mood improvement, and neurodegenerative disease models.

Chemical Properties and Specifications

Structural and Molecular Characteristics

• Chemical Formula: C22H30N4OC22H30N4O

• Molecular Weight: 366.5 g/mol

• CAS Number: 1270138-40-3

• IUPAC Name: (4-benzylpiperazin-1-yl)-[2-(3-methylbutylamino)pyridin-3-yl]methanone

• Synonyms: NSI-189 base, NSI-189 Free Base

Physical Properties and Purity Standards

• Appearance: Clear to slightly yellowish liquid solution

• Purity: Typically ≥98% pure NSI-189 in solution form

• Solubility: Highly soluble in solvents like DMSO and ethanol; provided pre-dissolved in solvent for ease of use

• Storage Recommendations: Store solution in a cool, dry, dark place, preferably refrigerated at 4°C or lower to maintain stability and prevent degradation

Pharmacological Profile and Mechanism of Action

Proposed Mechanism of Action

NSI-189 Free Base in solution retains the neurogenic mechanism typical of NSI-189 compounds. It promotes proliferation and differentiation of neural stem cells in the hippocampus, enhancing brain plasticity and volume. This action is independent of common neurotransmitter systems such as monoamine pathways, offering a novel approach to neurological research and potential therapeutic strategies.

Research Applications

NSI-189 Free Base Solution is used in in vitro and in vivo studies focusing on neurogenesis, neuroprotection, and cognitive function. The solution format enables precise dosing for cell culture experiments, animal studies, and other neuropharmacological research exploring major depressive disorder, cognitive impairment, and brain injury recovery.

Comparative Analysis with Related Compounds

Compare to Related Compounds

NSI-189 solution is often compared to the phosphate salt and powder forms of NSI-189. The solution offers superior convenience, immediate availability for dosing, and consistent concentration for experiments. Unlike other neurogenic agents like fluoxetine or ketamine, NSI-189 in solution specifically targets hippocampal neurogenesis rather than broader neurotransmitter systems.

Advantages for Research

The pre-made solution eliminates the need for solubilization steps with solvents like DMSO, reducing preparation time and variability. This ready-to-use format supports reproducible dosing and improved safety in laboratory handling, especially in sensitive biological studies.

Safety and Handling Guidelines

Laboratory Safety

Handle NSI-189 Free Base Solution using gloves, lab coat, and eye protection. Use in well-ventilated areas or biosafety cabinets to prevent inhalation of vapors. Avoid ingestion and skin contact.

Disposal and Environmental Considerations

Dispose of any unused solution and containers according to hazardous chemical waste guidelines, ensuring no release into the environment. Follow institutional and governmental regulations for chemical disposal.

Regulatory Status

NSI-189 Free Base in solution form is for research use only and is not approved for clinical or human consumption. Its distribution is controlled under relevant chemical and pharmaceutical research regulations.

Frequently Asked Questions (FAQs)

1. What concentration is NSI-189 Free Base Solution typically sold in?
   Usually around 40 mg/mL for research use.

2. How should NSI-189 solution be stored?
   Stored in a cool, dark place, ideally refrigerated at 4°C.

3. Is NSI-189 solution ready to use without dilution?
   Yes, it is typically prepared for direct experimental use or requires minimal dilution based on protocol.

4. Can NSI-189 solution be used in cell culture?
   Yes, it is commonly used for in vitro neurogenesis and neuroprotection studies.

5. Is the safety profile the same as NSI-189 powder?
   Yes, standard lab safety protocols apply equally.

Conclusion

NSI-189 Free Base Solution offers a convenient, ready-to-use liquid form of the neurogenic compound, facilitating research into neurogenesis, cognition, and neurological disorders. Its high purity, controlled concentration, and stability make it ideal for advanced neuroscience experiments under strict laboratory safety guidelines.